Gene name: | IGFBP6 |
Aging type: | Prevent |
Aging characteristic: | Others |
Tissue type: | -- |
Cell name: | HDF |
Experiment: | BrdU assay//SA-β-gal activity assay//Growth curve assay |
Description: | At the end of the lifespan, overexpression of IGFBP-6 enabled the cells to perform 4– 5 additional population doublings.This was accompanied by a significant increase in the cell proliferation rate as measured by BrdU incorporation and a significant decrease in apoptotic cell death determined by Annexin V-staining and propidium iodide staining at 90–95% of lifespan completed. 120 days after infection (ca. 95% of lifespan completed), nearly all the cells in the controls stained SA-β-gal positive, whereas roughly 50% of the IGFBP-6 expressing cells stained still SA-β-gal negative and displayed reduced p21Waf1/Cip1 protein expression relative to controls. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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