| Gene name: | CHK1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Gene ID: | 852577 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 20716119 |
| Experiment: | Knockdown//Flow cytometry//Immunofluorescence//SA-β-gal activity assay |
| Description: | A reduction in the cell numbers in the G1 or G2/M phases of HCT116 wt or p53–/–at 72 hrs following Chk1 siRNA transfection caused enhanced cell death reflected by increased Pre-G1 cell populations.cyclin B1 was found in the nucleus in 90% of p53–/–cells, allowing for the conclusion that p53–/– cells accumulated in late G2 just before mitosis .We found that wt cells establish a G1 arrest following Chk1-involved G2 arrest, which was associated with a senescent phenotype as shown by staining for β-galactosidase activity.In addition, wt cells showed the characteristic flattened and enlarged morphology in the majority of cells together with prolonged expression of p53 and p21WAF1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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