| Gene name: | BRG1 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Bone marrow |
| Cell name: | MSC |
| Experiment: | Knockdown//SA-β-gal activity assay//Flow cytometry |
| Description: | BRG1 silencing reduced the percentage of S-phase cells and induced a decrease in apoptosis,We observed a small increase of G1 in MSCs with silenced BRG1 compared with controls (67.61 versus 58.33%). It is noteworthy that MSCs with silenced BRG1 had a significant lower percentage of S-phase cells (Po0.05; 29.11 versus 40.23%) and an increase of G2/M cells (3.28 versus 1.43%).We observed signs of senescence in cells treated with Ad-siRNA-2405, as detected by acid-β-galactosidase, compared with cells transduced with control Ad-siRNA. |
| Regulatory pathway: | RB-P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | RB1-TP53 |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | In MSCs transduced with Ad-siRNA2405 or with control virus, we inhibited P53 with Ad-CMVE1A(YH47-928) and RB family proteins with AdCMV-E1A(RG2). Both RB and P53 seemed to have a role in BRG1-mediated senescence. In situ acid-bgalactosidase staining showed that in cells with silenced BRG1 the inhibition of P53 and of RB reduced the percentage of senescent cells . In contrast, protection from apoptosis seemed to rely mainly upon the RB family, because inactivation of these proteins significantly increased the percentage of apoptotic cells, reaching a percentage higher than that observed when BRG1 was silenced. |
Annotation:
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