| Gene name: | PTTG1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | IMR-90,BJ-1,WI-38 |
| Experiment: | SA-β-gal activity assay//Cell proliferation assay//Flow cytometry |
| Description: | The cell number of PTTG1- expressing cells did not increase after infection, indicating that PTTG1 inhibited proliferation of normal cells. Flow cytometry analysis revealed that the proportion of G1 cells was reduced in PTTG1-expressing cells.Our results clearly indicated that IMR90 cells expressing PTTG1 showed an induction of SA-β -gal activity. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Cell proliferation assay//Knockdown |
| Target gene description: | The level of PTTG1-activated p53 was decreased upon shRNA treatments. Significantly, the growth of PTTG1- expressing cells was increased upon knocking down p53 expression. The results indicated that reduced p53 function could restore PTTG1-induced growth inhibition in normal cells. The SA-β-gal activity was then checked to evaluate the effect of a lower p53 level on PTTG1-induced senescence. In the p53 knockdown cells, the number of SA-β-gal-positive cells was also significantly lower than that of the control cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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