| Gene name: | CENPA |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | TIG-3,HeLa |
| Experiment: | BrdU assay//Knockdown//Western blot//SA-β-gal activity assay |
| Description: | A BrdU incorporation assay (BrdU is a marker of DNA synthesis) demonstrated that CENP-A depletion in TIG3 cells reduced the proportion of cells transiting S phase of the cell cycle. The proportion of M-phase cells was also reduced in the CENP-A-depleted TIG3 cells, as determined by immunostaining for S10-phosphorylated histone H3 (a marker of late G2 and mitotic cells),immunoblotting analysis demonstrated that CENP-A depletion in TIG3 cells led to a reduction in the levels of cyclin B and CDC2, which are essential for cell cycle progression, and an elevation of the levels of the CDK inhibitors, p16INK4a and p21CIP1.TIG3 cells depleted of CENP-A also exhibited the increase in SA- β-Gal activity that is the cytological marker of senescent cells. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//Proliferation assay |
| Pathway description: | P53 appeared to be essential for this proliferation arrest; CENP-A depletion in p53-depleted TIG3 cells did not stop proliferating. |
Annotation:
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