| Gene name: | TRX1 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | BJ |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | As demonstrated by cessation of population doublings and by upregulated senescence-associated beta-galactosidase (SA-beta-gal) activity.Quantification of the SA-beta-gal staining showed that the percentage of cells with positive activity was significantly higher in cells infected with either of the shTRX1 constructs than for cells infected with the control shRNA construct against GFP. |
| Regulatory pathway: | P53//P16 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53//CDKN2A |
| Pathway experiment: | Western blot |
| Pathway description: | TRX1 suppression in BJ cells led to persistent upregulation of p53/p21 and p16INK4a proteins .TRX1 suppression in the BJELT cells prevented the proliferation defect observed in normal BJ cells with intact p53 and p16INK4a pathways , verifying that either one or both pathways have functional roles in establishing the shTRX1-induced senescent phenotype as opposed to the proliferation defect arising from quiescence or another non-senescence-related mechanism. |
Annotation:
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