Aging Overview

Aging gene

Gene name: NFE2L2
Aging type: Prevent
Aging characteristic: Others
Tissue type: --
Cell name: HFL-1
Gene ID: 4780
Category: protein coding
Phenotype: Aging
Experimental category: L
PMID: 20068043
Experiment: Knockdown//SA-β-gal activity assay//Cell proliferation assay//Western blot
Description: Knockdown of Nrf2 resulted in inhibition of proliferation and induction of premature senescence in HFL-1 fibroblasts.The cells acquired the typical senescent morphology, exhibited a higher percentage of β-galactosidase positive staining and revealed increased levels of p16 protein,as compared with the HFL-1 cells transfected with scrambled siRNA.


Regulatory relationship

Target gene: 18αGA
Official symbol(s): 18αGA
R-AG-Target gene: --
Subcategory: Unclear
Target gene experiment: qRT-PCR//Western blot//Immunoblotting
Target gene description: Specifically, treatment of HFL-1 young cells with 2 μg/ml of 18αGA for 2 h (optimal conditions, see next paragraph) induced both the RNA and protein expression levels of Nrf2. In support, we have observed reduced levels of Keap1 as well as increased levels of its modified isoform (a protein greater than 150 kDa). Moreover, cell fractionation experiments as well as immunofluorescence analysis under the same conditions revealed that treatment with 18αGA promoted Nrf2 translocation to the nucleus, a feature that concurs with its activation.

Regulatory pathway: --
R-AG-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



About risk SNP and eQTL

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