Gene name: | NFE2L2 |
Aging type: | Prevent |
Aging characteristic: | Others |
Tissue type: | -- |
Cell name: | HFL-1 |
Experiment: | Knockdown//SA-β-gal activity assay//Cell proliferation assay//Western blot |
Description: | Knockdown of Nrf2 resulted in inhibition of proliferation and induction of premature senescence in HFL-1 fibroblasts.The cells acquired the typical senescent morphology, exhibited a higher percentage of β-galactosidase positive staining and revealed increased levels of p16 protein,as compared with the HFL-1 cells transfected with scrambled siRNA. |
Target gene: | 18αGA |
Official symbol(s): | 18αGA |
R-AG-Target gene: | -- |
Subcategory: | Unclear |
Target gene experiment: | qRT-PCR//Western blot//Immunoblotting |
Target gene description: | Specifically, treatment of HFL-1 young cells with 2 μg/ml of 18αGA for 2 h (optimal conditions, see next paragraph) induced both the RNA and protein expression levels of Nrf2. In support, we have observed reduced levels of Keap1 as well as increased levels of its modified isoform (a protein greater than 150 kDa). Moreover, cell fractionation experiments as well as immunofluorescence analysis under the same conditions revealed that treatment with 18αGA promoted Nrf2 translocation to the nucleus, a feature that concurs with its activation. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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