| Gene name: | PPM1D |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | mesenchymal-stem-cell |
| Experiment: | XTT assay//Growth curve assay |
| Description: | Early-passage hMSCs (p5, open circle) and late-passage Wip1-hMSC (p12, filled square) proliferated normally, whereas late-passage Cont-hMSCs (p12, open square) underwent distinct cell growth arrest(To define ‘‘early-passage’’ and ‘‘late-passage’’ cells, we referred to the previous report that elucidated the growth kinetics of hMSCs [6]. According to those results, the growth of hMSCs is significantly retarded after passage number 10; thus, we used the term late passage for hMSCs grown more than 10 passages. This difference in cell growth rate was reproduced using the XTT assay: late-passage hMSCs (p13) barely proliferated after 1 week, whereas Wip1-hMSCs at the same passage level proliferated in a fashion similar to early-passage hMSCs.We therefore concluded that Wip1-hMSCs bypassed the cell growth arrest that occurs in late-passage hMSCs. |
| Target gene: | P16//P38 |
| Official symbol(s): | P16//P38 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Consistently, p16 mRNA levels determined by semiquantitative real-time PCR were upregulated in late-passage hMSCs, compared with early-passage hMSCs that had not experienced growth arrest. Interestingly, p16 mRNA levels in later passage Wip1-hMSCs were significantly lower than in the late-passage hMSCs.As expected, p38 activity determined by p38 in vitro kinase assay using ATF-2 as a substrate was increased by 50%, whereas p38 activity in Wip1-expressing hMSCs was decreased by 50%. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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