| Gene name: | TOP1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38,IMR-90,U2OS,HDF |
| Experiment: | SA-β-gal activity assay//Knockdown//Colony formation assay//Growth curve assay |
| Description: | Colony formation by Top1-depleted HDFs correlated with a loss of senescence markers: the formation of senescence-associated heterochromatin foci and the appearance of senescence-associated β-galactosidase activity.Top1 knockdown extended the life span of WI38 cells to f10 more population doublings,Growth of U2OS or WI38 cells constitutively expressing GFPTop1 was much slower than that of control-transfected U2OS cells. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Furthermore, measurement of p53 activity in the presence of an increasing amount of Top1 with a constant amount of a p53 activity reporter revealed dose-dependent activation of p53 by Top1. To further confirm the involvement of the p53 pathway, we examined the effect of p53 inhibition by a dominant negative form of p53 (p53DN) over the proliferation arrest induced by GFPTop1. Interestingly, p53 pathway inhibition reverted efficiently the proliferation arrest induced by GFPTop1. |
Annotation:
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