| Gene name: | CPEB1 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | HFF,WI-38 |
| Experiment: | SA-β-gal activity assay//Knockdown//Western blot |
| Description: | After 68 population doublings, the mock-infected and shTETR-infected cells stopped dividing and assumed a flat senescent-like morphology. These cells also stained for β-galactosidase activity at acidic pH, a common marker for senescence. The shCPEB-infected cells, however, continued to grow, did not undergo a morphology change, and did not stain for β-galactosidase activity. Moreover, while mock-infected and shTETR-infected cells expressed high levels of p21CIP1 and p16INK4A, which is consistent with entry into senescence, the cells infected with shCPEB did not. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Two days later, the cells were infected with a virus expressing CPEB; the cells were then analyzed for growth and p21, a target gene of p53.While CPEB induced senescence in cells lacking GSE-22, they were unable to do so if they contained the inhibitory peptide. Moreover, GSE-22 prevented p21 expression, thus demonstrating that it indeed inhibited p53 activity. These results indicate that CPEB-induced senescence requires p53 in human cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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