Aging Overview

Aging gene

Gene name: IFNG
Aging type: Accelerate
Aging characteristic: Others
Tissue type: --
Cell name: HUVEC,MEF
Gene ID: 3458
Category: protein coding
Phenotype: Atherosclerosis
Experimental category: L
PMID: 19071156
Experiment: Cell proliferation assay//Flow cytometry//SA-β-gal activity assay
Description: Cell proliferation decreased slightly with IFN-g treatment in time- and dose-dependent manners. A decrease in cell proliferation by IFN-g treatment (more than 1000 U/ml) for 3 days was statistically significant (p < 0.05).The induction of cellular senescence by prolonged treatment with IFN-g was confirmed by an increase in SA-β-gal-positive cells in the treated cells compared to the control cell. IFN-γ treatment increased the G0/G1 cell population and decreased the S and G2/M cell population, suggesting that IFN-γ inhibited cell proliferation through G0/G1 arrest in the cell cycle. No difference in cell cycle status was observed in IFN-a treated cells for 6 days.


Regulatory relationship

Regulatory pathway: P53
R-AG-Pathway: --
Official symbol(s): TP53
Pathway experiment: Knockdown//Western blot//SA-β-gal activity assay
Pathway description: Prolonged stimulation of IFN-g in the p16-knockdown (p16sh) cells increased SA-β-gal activity staining to a level similar to that in control cells. In contrast, no increase in SA-β-gal staining was observed in the p53-knockdown (p53sh) cells following prolonged stimulation with IFN-g. To obtain more evidence that IFN-g-induced senescence involves a p53 signaling pathway, we measured the effects of IFN-g on cellular senescence in wild, p16-/-, or p53-/- MEFs. We reproducibly noticed an increase in the number of SA-b-galpositive cells in p16-/- MEFs, but not in IFN-g-treated p53-/- MEFs, after prolonged stimulation with IFN-g. Therefore, these results suggest that IFN-g-induced cellular senescence is mediated through a p53-dependent pathway.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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