| Gene name: | IGFBP3 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Incorporation of [3 H]thymidine was estimated between 48 and 72 h after the last stress.A decreased proliferative potential of HDFs (pb0.001) was observed in t-BHP- and ethanoltreated cells compared to control cells (no siRNA). The presence of IGFBP-3 siRNA strongly attenuated the reduction of the proliferative potential of HDFs observed after treatment with either t-BHP (pb0.01) or ethanol (pb0.001).S-A β-gal was monitored using microfluidic detection at 72 h after the last stress.The increased proportion of S-A β-gal-positive HDFs in t-BHP- or ethanol-induced premature senescence was largely attenuated in HDFs transfected with IGFBP-3 siRNA. |
| Target gene: | TGF-β1 |
| Official symbol(s): | TGFB1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunofluorescence |
| Target gene description: | We detected an increase of more than 20-fold in the relative transcript level of IGFBP-3 as well as an increase in IGFBP-3 protein level by immunofluorescence semiquantitative confocal microscopy. Moreover, neutralizing antibody against TGF-β1 added in the medium after the last EtOH- or t-BHP stress inducing premature senescence diminished the increased transcript and protein levels of IGFBP-3. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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