| Gene name: | CDK4 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | SA-β-gal activity assay//Cell counting//BrdU assay//Western blot |
| Description: | Forced expression of wtCDK4 generated colonies in which growth was rapid for ~4 weeks then began to slow and finally ceased after ~7 weeks in a state resembling M1. Similar growth kinetics were observed for wtCDK6 colonies with a final average size of 7*103 cells (range: 103 to 4*104;n=25) corresponding to 12.5 PD and for mCDK6 colonies with a final colony size of 8*103 cells (range: 0.6*103 to 2.7*104; n=14)corresponding to ~13 PD. Colonies expressing mCDK4 di?ered slightly in that growth started to slow after 2 to 3 weeks.Cells were large and attened with a granular cytoplasm and could be maintained in a proliferatively quiescent but viable state for prolonged periods of time with occasional refeeding. SAβ-gal activity was high (>95% of cells positive by histochemical assay), BrdU LI was very low (<2%) (data not shown), and TdT assay confirmed that apoptotic cells in all MintCDK populations were extremely rare.Western blot analysis confirmed that growth arrest was not due to loss of expression of the exogenous CDK genes. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | p53 was elevated (12 ± 37-fold) in all CDK overexpressors compared to the pBABEpuro control. |
Annotation:
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