| Gene name: | TXNIP |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Kidney |
| Cell name: | MEF |
| Experiment: | Cell counting//Cell morphological analysis//SA-β-gal activity assay//Western blot//qRT-PCR |
| Description: | The growth rate of KO MEF cells was dramatically decreased, and the cells almost ceased to proliferate at late passages (P5).KO MEF cells were larger than WT MEF cells. KO MEF cells were stained with SA‐β‐gal and γ‐H2AX at Passage 5 (P5).The expression levels of g p53, p21, p16,PAI‐1, and PML are upregulated by western blotting and statistical analysis or quantitative real‐time PCR in KO MEF cells in comparision to WT MEF cells. |
| Regulatory pathway: | AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | AKT1 |
| Pathway experiment: | Co-IP//Western blot |
| Pathway description: | TXNIP was found to interact with AKT, which was also confirmed by immunoprecipitation assays in 293T cells.The expression of TXNIP inhibited the activity of AKT in a dose dependent manner in western blotting with phospho AKT antibodies and in vitro kinase assays. |
Annotation:
Loading,please wait...