| Gene name: | NANOG |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Hair follicle |
| Cell name: | HF-MSC |
| Experiment: | Cell counting//SA-β-gal activity assay |
| Description: | Cell growth assays showed that the ectopic expression of NANOG significantly increased the quantity of HF-MSCs by 1.4- and 1.2-fold,in comparison to cells in the vector group (control) on day 8.Ectopic NANOG expression significantly decreased the percentage of SA-β-gal-positive cells. |
| Target gene: | P16//P21//P53//PBX1 |
| Official symbol(s): | P16//P21//P53//PBX1 |
| R-AG-Target gene: | Downregulation//Downregulation//Downregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Luciferase reporter assay |
| Target gene description: | Western blotting showed that ectopic NANOG expression significantly upregulated PBX1 expression by 4.11±0.54-fold in comparison to that of the control group. Dual-luciferase reporter gene assays showed that ectopic NANOG expression significantly increased PBX1 promoter activity by 1.99±0.43-fold incomparison to that of the control group (P < 0.05). HNANOG decreased p16 expression by 0.38±0.18-fold (P < 0.01),p21 expression by 0.57±0.24-fold (P < 0.05) and p53 expression by 0.60±0.16-fold (P < 0.05). |
| Regulatory pathway: | AKT |
| R-AG-Pathway: | Activation |
| Official symbol(s): | AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | Western blotting showed that NANOG significantly increased PARP1 expression by 1.64 ± 0.09-fold (P < 0:01) and the expression of p-AKT/AKT by 3.14 ± 0.65-fold (P < 0:05),in comparison to the empty vector. |
Annotation:
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