| Gene name: | SPIN1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | HeLa |
| Experiment: | PI staining//Flow cytometry//DAPI staining//CCK-8 assay//SA-β-gal activity assay |
| Description: | SPINDLIN1 over-expressing cells showed a heterogeneous DNA content, with broader G1 and G2 peaks and a substantial fraction of cells with 4N DNA content, at 29 days after transduction.Numerous large cells with multiple nuclei were easily visible under phase contrast microscope, and multinucleation was also clearly illustrated by DAPI staining .HeLa cell growth was delayed significantly by overexpression of SPINDLIN1.We indeed found that almost all adherent multinucleated cells were SA-βgal positive (3.2%( 1.4%) in SPINDLIN1 overexpressing cells verse 0.8% ( 0.3%) in mock transduction cells). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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