| Gene name: | HSPB2 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MCF 10A |
| Experiment: | Colony formation assay//Cell morphological analysis//SA-β-gal activity assay |
| Description: | Hsp27 overexpression led to a significant increase in survival after doxorubicin treatment. significantly fewer Hsp27-MCF10A cells showed expression of acidic β-galactosidase and senescence morphology after treatment with doxorubicin, compared with control MCF10A cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | We observed a suppression of known downstream targets of p53, survivin, and cdc2 in shHsp27 cells.In fact, direct measurement of the degradation rates showed that the half-life of p53 in shHsp27 cells increased from 26to 42 min .Doxorubicin led to a pronounced accumulation of p21 in control cells but this increase was markedly suppressed in Hsp27-MCF10A cells.Nutlin-3 treatment for 48 h led to accumulation of p21 in control cells, but this accumulation was suppressed in Hsp27-overexpressing cells. |
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