| Gene name: | KL |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown |
| Description: | Downregulation of Klotho expression by RNAi significantly shortened replicative lifespan and reduced the growth rate of MRC-5 cells compared with mock-infected cells.Similar to the phenotype of senescent cells, Klotho RNAi cells were flattened and enlarged .we found a 15-fold increase in β-Gal activity in Klotho RNAi (1)-transduced cells compared to mock control and a sixfold increase for Klotho RNAi (2). |
| Regulatory pathway: | INSULIN-IGF-1//P53-P21 |
| R-AG-Pathway: | --//Downregulation |
| Official symbol(s): | INS-IGF1//TP53-CDKN1A |
| Pathway experiment: | Western blot//SA-β-gal activity assay |
| Pathway description: | The level of phosphorylated IRS1 is higher in MRC-5 cells treated with Klotho RNAi, suggesting an upregulation of signaling at this early step in the pathway. the levels of phosphorylated AKT and phosphorylated FOXO3 (the inActivate cytoplasmic form) are higher.Relative to control cells Klotho RNAi infected cells have similar levels of p16 protein but elevated expression of p21.Confirming the ability of p53 attenuation to restore replicative potential of Klotho RNAi cells, staining for β-Gal shows approximately the same number of positive cells in the double knockdown compared to the vector control or p53 RNAi cells . |
Annotation:
Loading,please wait...