| Gene name: | PIAS4 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | Growth curve assay//Cell morphological analysis//SA-β-gal activity assay//Immunostaining//Flow cytometry//SAHF |
| Description: | We found that cells constitutively expressing PIASy irreversibly arrested growth 7 10 days postselection, whereas other PIAS proteins had no effect available with this article online).PIASy-overexpressing cells also exhibited a flat cell morphology, and more than 80% of the cell population stained SA-β-gal positive.These assays clearly demonstrated that PIASy-expressing cells were completely devoid of proliferative potential and predominantly arrested in G1 .We also observed formation of SAHFs in PIASy-expressing cells. |
| Target gene: | E6 |
| Official symbol(s): | E6 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Addition of PIASy, but not of other PIASs, to the reaction strongly enhanced the formation of HDAC1-SUMO conjugates, an effect that was abrogated by the presence of E6 or its 45/47/49 mutant.11E6 was also unable to inhibit PIASy-induced senescence, whereas E6 mutant 45/47/ 49 still did. |
| Regulatory pathway: | P53-P21//RB-P16 |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | TP53-CDKN1A//RB1-CDKN2A |
| Pathway experiment: | Western blot |
| Pathway description: | These data corroborate the increased p21 protein levels we observed in PIASy-transduced fibroblasts and suggest that sumoylation might contribute to PIASy-mediated activation of p53 in the context of PIASy-dependent senescence.PIASy corepressed the cyclin E promoter in an Rb- and dosedependent manner.Loss of PIASy using siRNA in SAOS-2 cells caused a significant derepression of the cyclin E promoter in the presence of Rb. |
Annotation:
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