| Gene name: | MYC |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | BJ,IMR-90 |
| Experiment: | Colony formation assay |
| Description: | We introduced into the c-myc+/- cells a retrovirus vector expressing human telomerase reverse transcriptase (hTERT) to immortalize them. Although hTERT clearly extended their lifespan, several attempts with different vectors failed to elicit long term immortalization, whereas the same vectors readily immortalized c-myc+/+ cells in parallel experiments. |
| Target gene: | BMI-1//P16 |
| Official symbol(s): | BMI-1//P16 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//CHIP |
| Target gene description: | As expected, retrovirus- mediated overexpression of c-Myc in normal HDFs resulted in Bmi-1 mRNA induction.we demonstrated direct binding of c-Myc protein to the E-box in the bmi-1 promoter by chromatin immunoprecipitation (ChIP) analysis.In the absence of ectopic Bmi-1, lentivirus vector-expressed c-Myc shRNA elicited a 2-fold up-regulation of p16 mRNA within 3 days of infection. Ectopic Bmi-1 expression alone resulted in repression of p16 mRNA levels, which remained low after c-Myc knockdown.In all cases, down-regulation of c-Myc caused the down-regulation of Bmi-1 and the concomitant up-regulation of p16. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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