| Gene name: | ING2 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | Western blot//SA-β-gal activity assay//Colony formation assay//Crystal violet assay |
| Description: | ING2 protein level was four- to sixfold higher in senescent fibroblasts.ING2 overexpression induced premature senescence in PDL28 fibroblasts. The downregulation of ING2 resulted in a statistically significant increase only in the number of small colonies (containing 10 to 50 cells). Thus, suppression of ING2 expression results in an extension of the life span of human fibroblasts for a limited number of divisions. |
| Target gene: | P53//P300 |
| Official symbol(s): | P53//P300 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining//Co-IP//Western blot |
| Target gene description: | The colocalization of ING2 with p53 (Ser15) was dramatically enhanced in senescent fibroblasts.The complex formation between p53, ING2, and p300 was further corroborated in transient transfections in wild-type p53-containing U2OS cells, where immunoprecipitation of endogenous p300 also contained p53 and transfected Flag-ING2.With a constant amount of p300, increasing the amounts of purified ING2 protein led to a further two- to fourfold increase in the acetylation of lysine 382 on p53, as determined by the two detection methods. No p53 acetylation was detected with ING2 protein alone. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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