| Gene name: | LEO1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | MTT assay//Immunostaining//Flow cytometry//Cell morphological analysis//SA-β-gal activity assay |
| Description: | 2BS/Leo cells showed complete growth inhibition similar to senescent cells (PD56).In contrast to the significantly increased G1 DNA content of 2BS/Leo, which was somewhat like senescent cells, RDL-Leo–reduced the G1 content of transfected cells.2BS/Leo cells showed increasing gross enlargement,flattening, and accumulation of granular cytoplasmic inclusions, like senescent cells (PD56).No SA-β-Gal activity was observed in 2BS/RDL-Leo–(PD48) and young (PD 27) cells, whereas almost all 2BS/Leo(PD 48) cells were strongly stained, as were senescent 2BS control cells (PD 56). In contrast, 2BS/Leo ceased cell division 7–12 PD (CPD 48-50) earlier than did normal cells. |
| Regulatory pathway: | P16//P21//PTEN |
| R-AG-Pathway: | Upregulation//Upregulation//Upregulation |
| Official symbol(s): | CDKN2A//CDKN1A//PTEN |
| Pathway experiment: | Western blot |
| Pathway description: | The results showed that introduction of Leo into 2BS cells affected all three pathways by increasing the expression of p16INK4a, p21WAF1, and PTEN. |
Annotation:
Loading,please wait...