| Gene name: | DHCR24 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | REF,WI-38 |
| Experiment: | Cell morphological analysis//BrdU assay//SA-β-gal activity assay |
| Description: | In contrast, upon Ras introduction, REF cells with Seladin-1 knockdown continued to proliferate, exhibited a condensed cell morphology, and were not stained in SA-β-gal assay .We observed that cells with Seladin-1 knockdown continued to show BrdU incorporation, cell proliferation and approximately fourfold less SA-β-gal staining after H2O2 treatment. |
| Target gene: | P53//MDM2 |
| Official symbol(s): | P53//MDM2 |
| R-AG-Target gene: | Inhibition//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Silencing of Seladin-1 prevented Ras-induced p53 accumulation. We found that, first, Seladin-1 binds p53 and displaces Mdm2 from p53 in vitro.Second, recombinant Seladin-1 inhibits in vitro ubiquitination of Flag-p53 by Mdm2.Recombinant Seladin-1 inhibits in vitro ubiquitination of Flag-p53 by Mdm2 (Fig. 4d). |
| Regulatory pathway: | RAS |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | KRAS |
| Pathway experiment: | Western blot |
| Pathway description: | Seladin-1 levels increased in response to Ras expression. |
Annotation:
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