| Gene name: | NINJ1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | HCC tissue,Liver |
| Cell name: | HuH-7 |
| Gene ID: | 4814 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 15464245 |
| Experiment: | Flow cytometry//SA-β-gal activity assay//Autofluorescence |
| Description: | Injurin1 overexpression increased the percentage of the total cell population in the G0–G1 phase, decreased the percentage of cells in the S phase. The percentage of SA-β-galactosidase-positive cells in ninjurin1-overexpressing populations was 26% in Nin1 and 21% in Nin2. In contrast, less than 1% of the parental and mock clones were SA-β-galactosidasepositive.Nin1 and Nin2 cells displayed intense cytoplasmic, granular fluorescence.Flow cytometric analysis revealed that both Nin1 and Nin2 cells showed a discernable increase in fluorescence in comparison to the mock clones. |
| Target gene: | P21//CYCLIN A//CDK2//CDK4//CDK6 |
| Official symbol(s): | P21//CYCLIN A//CDK2//CDK4//CDK6 |
| R-AG-Target gene: | Upregulation//Downregulation//Downregulation//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | There was no change in the level of p27, but the level of expression of p21 was greatly upregulated.The level of expression of cyclinA declined.The levels of expression of all CDKs(CDK2,CDK4,CDK6) were consistently suppressed by ninjurin1 overexpression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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