| Gene name: | MAP2K1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Small intestine |
| Cell name: | HIEC,IEC-6 |
| Experiment: | Cell counting//Western blot//Cell morphological analysis//SA-β-gal activity assay |
| Description: | By contrast, caMEK-expressing HIEC cells did not proliferate and stopped accumulating well before reaching confluence.Ectopic expression of caMEK significantly inhibited thymidine kinase gene expression by 50%.Morphological examination of HIEC cells arrested by caMEK revealed that cells remained attached to the plates for at least 1 mo (data not shown) and acquired a large and flat morphology.Moreover, these HIEC cells accumulated SA-β-galactosidase. |
| Regulatory pathway: | MEK-ERK |
| R-AG-Pathway: | -- |
| Official symbol(s): | MAP2K7-MAPK1 |
| Pathway experiment: | Western blot |
| Pathway description: | Serum stimulation of HIEC and IEC-6 cells exhibited rapid and maximal activation of ERK1/2 within 10 min and persisted for up to 2 h. Nonstimulated caMEK expressing HIEC and IEC-6 cells showed a moderately increased basal level of ERK1/2 activities compared with pLXIN- and wtMEK-expressing cells. |
Annotation:
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