| Gene name: | MXD4 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | 293,HeLa |
| Experiment: | Colony formation assay//Growth curve assay//SA-β-gal activity assay//Western blot |
| Description: | Growth was repressed by about 4- and 2.5-fold in 293- and HeLa-hMad4 infected cells, respectively. This repression of cell proliferation was accompanied by an increase in contact inhibition in a soft agar colony assay.In the case of hMad4-infected cells, following a small and very slow proliferative phase from day 1 to day 26 following replating, cells completely stopped dividing at which point they acquire the morphological features of replicative senescence such as flat and enlarged morphology.hMad4-infected cells did express s-β-galactosidase expression while empty-vector- and hMad4L-P16-infected cells had a very low expression level.Only hMad4- infected cells had a high expression level of Pai-1 protein(senescence marker). |
| Target gene: | MAX//C-MYC |
| Official symbol(s): | MAX//C-MYC |
| R-AG-Target gene: | --//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Co-IP//CAT assay |
| Target gene description: | Anti-Max and antihMad4 are able to co-immunoprecipitate hMad4 and Max, respectively.When c-Myc and Max were co-transfected in 293 cells, there was a 10-fold increase in CAT activity over cells transfected with the reporter construct alone. When hMad4 was co-transfected with c-Myc and Max at increasing amount, there was a 4-fold decrease in CAT activity, similar to the activity seen with transfection of c-Myc alone. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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