| Gene name: | MAP2K6 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | U2OS |
| Experiment: | Colony formation assay//BrdU assay//Flow cytometry//SA-β-gal activity assay//Luciferase reporter assay |
| Description: | Relative to cells transfected with empty vector alone or with MKK6KA, ＜6% of the MKK6EE-transfected cells formed colonies.MKK6EE expression leads to a near complete loss of BrdUrd-positive cells, consistent with a G1/G0cell cycle arrest.We observed that 40% of MKK6EE-expressing cells are strongly positive for SA-βgal, consistent with a senescent phenotype.At 6 days after transfection ,lipofuscin was markedly increased in fluorescence of MKK6EE-expressing cells compared with controls. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Trikingly,p21Cip1, a cdk inhibitora markediy increase. |
| Regulatory pathway: | P38 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | MAKP14 |
| Pathway experiment: | SA-β-gal activity assay//Luciferase reporter assay |
| Pathway description: | As expected, expression of MKK6EE in U2OS cells increased the activity, whereas kinase-dead MKK6 slightly reduced basal activity .Cellular Senescence was not observed with MKK6KA cells or MKK6EE-expressing cells, which were treated with SB202190. |
Annotation:
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