| Gene name: | CDKN2B |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | U-251 MG,Tp483 MG,U373 MG,SW1783 |
| Experiment: | Cell counting//Cell morphological analysis//SA-β-gal activity assay//Telomerase Assay |
| Description: | Both inhibitors(p15 ,p16) were equally effective in causing complete growth arrest during a 7-day period.When U251 MG cells infected with Ad5CMVp15 or Ad5CMVp16 were stained for the SA-β-gal marker 8 days p.i., the morphologically changed, enlarged, and flattened cells were strongly positive in contrast to mock-infected or to Ad5CMVlacZ infected cells .In contrast, telomerase activity was efficiently repressed in both Ad5CMVp15- and Ad5CMVp16- infected cells, reaching an undetectable level at day 6. |
| Regulatory pathway: | RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Cell morphological analysis//SA-β-gal activity assay |
| Pathway description: | U373 MG cells infected with Ad5CMVp15 or Ad5CMVp16 showed no morphological changes even after 7 days.The U373 MG cell line(absent pRB expression) did not become SA-β-gal-positive after infection by adenoviruses expressing p15 or p16. |
Annotation:
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