| Gene name: | E2F1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38,NIH-3T3 |
| Experiment: | SA-β-gal activity assay |
| Description: | Wild-type E2F1, when constitutively overexpressed, unexpectedly arrested cell proliferation within two population doublings (PD) after selection.wild-type and D423G proteins, but not the other E2F1 proteins, also induced .50% of the cells to assume a flat morphology, typical of senescent cells. |
| Target gene: | P14//P53 |
| Official symbol(s): | P14//P53 |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Semi-RT-PCR//Western blot |
| Target gene description: | E2F1 proteins that had transactivation activity (wild type and D423G), but not the other E2F1 proteins, strongly induced p14ARF mRNA.p14ARF was also highly expressed by replicatively senescent cells.p14ARF and E2F1 did not arrest growth or induce SA-β-Gal staining in cells that overexpress Mdm2(Mdm2 functionally inactivates p53).Indeed, wild-type E2F1 and D423G proteins, as well as the p14ARF protein, strongly induced p53. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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