Aging Overview

Aging gene

Gene name: RAF1
Aging type: Accelerate
Aging characteristic: Others
Tissue type: --
Cell name: IMR-90
Gene ID: 5894
Category: protein coding
Phenotype: Aging
Experimental category: L
PMID: 9765202
Experiment: Cell counting//SA-β-gal activity assay
Description: Activation of GFPDRaf-1:ER arrested the proliferation of IMR-90 cells.Activation of GFPDRaf-1[YY]:ER in IMR-90 cells elicited the expression of acidic β-galactosidase, which was apparent within 2 3 days and maximal by 5 6 days after addition of 4-HT.


Regulatory relationship

Target gene: P16
Official symbol(s): P16
R-AG-Target gene: Upregulation
Subcategory: Unclear
Target gene experiment: Western blot//SA-β-gal activity assay//BrdU assay//Flow cytometry
Target gene description: Following activation of GFPDRaf-1:ER, we observed induced expression of the CDK inhibitors p16Ink4a.However, the level of expression of p16Ink4a did not decrease after GFPDRaf-1:ER inactivation, which may reflect the long half-life of both p16Ink4a mRNA and protein.IMR-90 cells expressing p16Ink4a became positive for SA–β-galactosidase activity.FACs can analysis revealed a threefold reduction in S-phase cells compared with the control infected populations.

Regulatory pathway: MEK-MAPK
R-AG-Pathway: Activation
Official symbol(s): MAP2K7-MAPK1
Pathway experiment: Western blot
Pathway description: As described above, activation of GFPDRaf-1[YY]:ER led to the phosphorylation and activation of the p42/p44 MAP kinases. In the presence of PD, however, phosphorylation of the MAP kinases was reduced to levels comparable to those found in uninduced IMR-90 cells.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

Loading,please wait...