| Gene name: | TP53 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | EJ |
| Experiment: | Cell morphological analysis//Flow cytometry//PI staining//BrdU assay//SA-β-gal activity assay |
| Description: | Proliferation of EJ–p53 cells overexpressing wt p53 (2tet) was almost completely inhibited.Tet removal led to obvious p53 nuclear staining by 24 h with the cells exhibiting increased size and a flattened morphology with elongated cellular processes, as well as enlarged nuclei.EJ–p53 cells exhibited greatly reduced BrdUrd incorporation within 24 h after the removal of tet with the fraction in S phase declining to ~5% by 2–3 days .Greater than 95% of such cells showed positive staining for SA-β-gal, which colocalized to cells possessing the senescent morphology . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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