| Gene name: | VEGFA |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Foreskin |
| Cell name: | HDMEC |
| Experiment: | Cell morphological analysis//Cell counting//SA-β-gal activity assay |
| Description: | When cultures were supplemented with a single pulse of VPF/VEGF at a final concentration of 5 ng/ml at the time of each passage after PD26, many HDMECs continued to maintain a relatively small size even beyond PD56 and continued to proliferate.When VPF/VEGF was withdrawn HDMEC developed a senescent morphology within 7 days. Addition of VPF/VEGF to senescent PD36 HDMEC induced renewed cell proliferation and restored a more normal morphology.senescent cells subsequently cultured with VPF/VEGF reduced their expression of neutral β-galactosidase. |
| Target gene: | P21//P16//P27 |
| Official symbol(s): | P21//P16//P27 |
| R-AG-Target gene: | Downregulation//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | p16 and p21 were only weakly expressed in early passage HDMEC(lane 3)but both were strongly expressed in senescent cells (lane 2). The expression of both was markedly reduced when senescence was prevented by culture with VPF/VEGF (lane 4).p27/Kip1 was moderately expressed in senescent HDMEC but at very low or undetec -table levels in both early passage cells cultured without VPF/VEGF and in late passage cells cultured with VPF/VEGF. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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