| Gene name: | CBX8 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MEF,Fibroblast |
| Experiment: | Flow cytometry//BrdU assay//Colony formation assay |
| Description: | We found that inhibition of CBX8 expression dramatically reduced the S-phase fraction of cells and led to a complete growth arrest.Overexpression of CBX8 bypassed senescence, and the growth rate of the CBX8 expressing cells did not change within the time frame of the experiment.We found that Cbx8 downregulation led to a significant reduction in the number of colonies in all the genetic backgrounds tested. |
| Target gene: | BMI1//INK4A//ARF |
| Official symbol(s): | BMI1//INK4A//ARF |
| R-AG-Target gene: | --//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//IP//qRT-PCR |
| Target gene description: | Western blot (WB) analysis of protein extracts before depletion (BD) and after depletion (AD) showed a reduction of about 60% of total CBX8 after BMI1 depletion and a reduction of about 20% of total BMI1 after CBX8 depletion.Consistent with binding to the Ink4a-Arf locus, CBX8 expression led to a decrease in Ink4a and Arf mRNA levels. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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