| Gene name: | BRAF |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Skin,Foreskin |
| Cell name: | Melanocyte |
| Experiment: | BrdU assay//SA-β-gal activity assay//SAHF |
| Description: | Short-term expression of BRAFE600(3–7 days) led to enhanced melanocyte proliferation that was measured by a moderate but reproducible increase in 5-bromodeoxyuridine (BrdU) incorporation. However, this effect was transient—sustained expression of BRAFE600resulted in marked cell cycle arrest.In most (roughly 80%) of the transduced melanocytes, this was associated with an intense activity of SA-β-Gal, a marker for senescent or stressed cultured cells as well as for aged tissues in vivo6,15.We observed that low levels of BRAFE600also induced SAHF. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining |
| Target gene description: | We found that BRAFE600-expressing melanocytes had elevated levels of p16INK4a. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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