| Gene name: | AGT |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Aorta |
| Cell name: | VSMC |
| Gene ID: | 183 |
| Category: | protein coding |
| Phenotype: | Atherosclerosis |
| Experimental category: | L |
| PMID: | 16908765 |
| Experiment: | SA-β-gal activity assay |
| Description: | SA-β-gal activity was significantly increased in Ang II–treated VSMCs compared with control cells.Ang II also significantly increased the transcriptional activity of p53 compared with that in vehicle-treated cells, and its effect was dose dependent.Consistent with our in vitro data, treatment with Ang II enhanced SA-β-gal activity in the aortas of apoE-deficient mice. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Knockdown//SA-β-gal activity assay |
| Pathway description: | Expression of p21 and p53 was elevated in Ang II–treated VSMCs.Western blot analysis revealed that introduction of E6 effectively reduced the level of p53 protein and also markedly reduced the expression of p21, its target protein.Overexpression of p21 significantly increased the number of SA-β-gal positive cells, whereas p21 knockdown with a small interfering RNA (siRNA) system effectively blunted the effect of Ang II on senescence. |
Annotation:
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