| Gene name: | SETD1A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Mammary Gland,Lung |
| Cell name: | MDA-MB-231,MCF-7,MDA-MB-468,BT-549,HT1299,A549 |
| Experiment: | SA-β-gal activity assay//Cell cycle analysis//Knockdown |
| Description: | Remarkably, knockdown of SETD1A(SETD1A-KD) suppresses proliferation and triggers prompt (72h) and massive cellular senescence, with very large cells expressing characteristic ?--galactosidase (?-gal) activity. The cell cycle arrest resulting from SETD1A-KD is consistent with cellular senescence, and it is not associated with apoptosis as seen by the absence of the caspase-3 and PARP cleavage markers of apoptosis, following 3 and 7-days of SETD1A-KD23,24. |
| Target gene: | SKP2 |
| Official symbol(s): | SKP2 |
| R-AG-Target gene: | -- |
| Subcategory: | Methylation |
| Target gene experiment: | Western blot//qPCR//SA-β-gal activity assay |
| Target gene description: | Inducible expression of SKP2 in SETD1A-KD cells(using two different shSET1A constructs) effectively reduced the expression of p27 and p21 proteins.Staining for ?-Galactosidase shows that induction of SKP2 partially suppressed the emergence of ?-Gal-positive senescent cells by 50% following SETD1A-KD. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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