| Gene name: | TIMELESS |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | The percentage of senescent cells identified by SA-β-gal staining is significantly increased in response to RasG12Vin 2BS human diploid fibroblasts and the percentage of cells with EdU incorporated is decreased.The cellular senescence model was further confirmed by Western Blot analysis of increased expression of p53, p21 and p16. |
| Target gene: | E2F1 |
| Official symbol(s): | E2F1 |
| R-AG-Target gene: | -- |
| Subcategory: | Binding to promoter |
| Target gene experiment: | CHIP |
| Target gene description: | Chromatin immunoprecipitation (ChIP) assay was utilized to confirm the binding of E2F1 to the endogenous TIMELESS promoter. Interaction between E2F1 and TIMELESS promoter region was detected by qRT-PCR. As expected, interaction between E2F1 and TIMELESS promoter was observed in young 2BS cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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