| Gene name: | PIM1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS,IMR-90 |
| Experiment: | SA-β-gal activity assay//CCK-8 assay |
| Description: | In the meanwhile, 2BS cells and IMR90 cells with UHRF1 knockdown exhibited lower cell proliferation rates and reduced EdU incorporation when compared with corresponding control groups. Furthermore, UHRF1 depletion also increased senescenceassociated β-galactosidase (SA-β-gal) activity in both cell lines. All of these data point to the same notion that knockdown of UHRF1 may induce premature senescence in human diploid fibroblasts. |
| Target gene: | UHRF1 |
| Official symbol(s): | UHRF1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | LC–MS/MS analysis//IP//Western blot |
| Target gene description: | The eluted protein complex was then resolved on sodium dodecyl sulfate–polyacrylamide gel electrophoresis, silver stained and subjected to LC–MS/MS analysis.The identified proteins were listed,including UHRF1, as well as other known interacting proteins of PIM1 such as Hsp9066and SND1,67suggesting that UHRF1 is a potential target of PIM1.Co-immunoprecipitation of UHRF1 followed by western analysis indicated that PIM1 associated with UHRF1 irrespective of whether the PIM1 kinase was wild-type or inActivate mutant. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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