| Gene name: | BRCA1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Mammary Gland |
| Cell name: | HMEC |
| Experiment: | Western blot//SA-β-gal activity assay |
| Description: | Western blot analysis of p16INK4a, total p53, p53 (Ser15) and p21 levels in WT and BRCA1mut/tHMECs at indicated PDs. M0, stasis, Ag, agonescence(WT HMECs), M*, premature growth arrest (BRCA1mut/tHMECs). Cells in both M* and Ag displayed the senescent phenotype, characterized by enlarged, flattened morphology and positive staining for SA-β-galactosidase. |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay |
| Target gene description: | Furthermore, knockdown of SIRT1 in WT HMECs resulted in cell-cycle arrest and morphological changes associated with senescence. |
| Regulatory pathway: | P53//PRB |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | TP53//retinoblastoma |
| Pathway experiment: | Western blot |
| Pathway description: | We found that this proliferative barrier was associated with elevated levels of all components of the p53 signalling pathway (phosphorylated p53 (Ser15), total p53, p21, p27). Collectively, these data indicate that activation of the pRb pathway is the primary mediator of HIS in BRCA1mut/t epithelial cells, and when bypass of HIS is forced (via down-regulation of pRb), it results in the activation of the p53 pathway and accumulation of additional genomic abnormalities. |
Annotation:
Loading,please wait...