| Gene name: | SPRTN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LCL |
| Gene ID: | 83932 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 25261934 |
| Experiment: | DNA fiber assay//Western blot//RNA-seq |
| Description: | To further confirm that mutations in SPRTN are the cause of the DNA replication defect, we transfected patients’ cells with WT SPRTN, which almost completely corrected the replication defect and restored cellular proliferation. siRNA-mediated SPRTN depletion severely affected the progression of DNA replication and induced an increased number of stalled forks, newly fired origins and formation of DSBs in the S phase of the cell cycle. Further, short interfering RNA (siRNA)-mediated depletion of SPRTN in HEK293T and U2OS human cell lines also caused chromosomal instability and severe proliferation defects, respectively. sprtn silencing, verified by a GFP reporter assay, led to a substantial increase of γ-H2AX foci, indicating an evolutionarily conserved functional role of SPRTN in the DNA damage response. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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