| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Experiment: | Immunofluorescence |
| Description: | SIRT1 depletion led to an increase in partially condensed, disorganized mass of chromosomes that failed to align properly on metaphaseplate in the presence of relatively normal spindles. |
| Target gene: | HISTONE 1(H1)//CONDENSIN I COMPLEX |
| Official symbol(s): | HISTONE 1(H1)//CONDENSIN I COMPLEX |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Protein gel blot analysis from asynchronous cell culture shows the reduction of the chromatin-bound condensin component CAp-D2 and histone H1 in cells treated with SIRt1 siRNA but not control siRNA.The levels of chromatin-bound histione H1 were significantly reduced in cells transfected with anti-SIRT1 siRNA. However, the levels of chromatin-bound CAP-D2, which is a condensin I component, were significantly reduced in cells transfected with anti-SIRT1 siRNA despite unchanged levels in nuclear soluble fraction. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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