| Gene name: | BCL6 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | Consistent with the ability of p53 to induce a G1 arrest, BCL6-transduced WI-38 cells exhibited an increase in the G1 phase fraction from 74 versus 42% in control cells; and a progressive reduction in their proliferative rate that could not be overcome by increasing serum concentration or passage in culture (data not shown). Also, certain morphological features, such as cytoplasmic vacuoles and enlarged cell size and flattening were observed. These changes were suggestive of cellular senescence, which was further confirmed by detection of pH-dependent β-galactosidase levels . Altogether, within 8 days, 37.5% of BCL6-transduced WI-38 cells displayed evidence of senescence as compared with 6% of GFP transduced or untransduced cells. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qPCR//Western blot |
| Target gene description: | However, just as in fibroblasts, BCL6 transduction powerfully induced TP53 mRNA and protein up-regulation as well as P21.p53 protein levels were correspondingly increased in the presence of BCL6. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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