| Gene name: | ALOX15B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Pca |
| Experiment: | SA-β-gal activity assay//BrdU assay//Cell morphological analysis |
| Description: | We followed PC3 cells that had been stably transfected with 15-LOX2 or 15-LOX2sv-b for multiple passages. These cells were derived from clonal cultures and enough cells generally became available for analysis only at P4–P5. To our surprise, these cells also showed passage-related phenotypic changes resembling replicative senescence in NHP cells. For instance, most of the early-passage cells were generally small, Activately proliferating, and SA-βgal-negative (not shown). By P6, 10–15% of the stably transfected cells became big and flat, some of which were also SA-βgal + and most of which were BrdU (not shown). With increasing passage, the percentage of big/flat cells also increased in both 15-LOX2- and 15-LOX2sv-b-expressing PC3 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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