Gene name: | CLPP |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | HepG2,HuH-7 |
Gene ID: | 8192 |
Category: | protein coding |
Phenotype: | Decompensated cirrhosis |
Experimental category: | L |
PMID: | 30878663 |
Experiment: | SA-β-gal activity assay//Flow cytometry//Immunostaining//Western blot |
Description: | Both HepG2 and Huh7 cells were transfected with either CLPP-GFP or GFP alone and stable cell clones were selected showing more than 90% GFP positivity.Unlike the control cells which showed a pan GFP expression, the CLPP over-expressing cells showed a punctate pattern in the cytoplasm which colocalized with Mitotracker, thereby confirming its mitochondrial localization.The growth kinetics of CLPP over-expressing cells was similar to their control counterpart. When treated with doxorubicin, the CLPP-GFP cells showed almost 50% reduction in SA-β-Gal staining when compared to control GFP cells. This was also accompanied with increased levels of LaminB1 and decreased levels of γH2AX and H3K9Me3 in doxorubicin treated CLPP cells.Senescence is often accompanied by a secretory phenotype which was much subdued in CLPP expressing cells when exposed to doxorubicin. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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