Aging Overview

Aging gene

Gene name: CLPP
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: HepG2,HuH-7
Gene ID: 8192
Category: protein coding
Phenotype: Decompensated cirrhosis
Experimental category: L
PMID: 30878663
Experiment: SA-β-gal activity assay//Flow cytometry//Immunostaining//Western blot
Description: Both HepG2 and Huh7 cells were transfected with either CLPP-GFP or GFP alone and stable cell clones were selected showing more than 90% GFP positivity.Unlike the control cells which showed a pan GFP expression, the CLPP over-expressing cells showed a punctate pattern in the cytoplasm which colocalized with Mitotracker, thereby confirming its mitochondrial localization.The growth kinetics of CLPP over-expressing cells was similar to their control counterpart. When treated with doxorubicin, the CLPP-GFP cells showed almost 50% reduction in SA-β-Gal staining when compared to control GFP cells. This was also accompanied with increased levels of LaminB1 and decreased levels of γH2AX and H3K9Me3 in doxorubicin treated CLPP cells.Senescence is often accompanied by a secretory phenotype which was much subdued in CLPP expressing cells when exposed to doxorubicin.


Regulatory pathway: --
R-AG-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



About risk SNP and eQTL

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