Gene name: | TERF2 |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | -- |
Cell name: | AG06814,GM00319,AG00780,AG03141 |
Gene ID: | 7014 |
Category: | protein coding |
Phenotype: | Werner syndrome |
Experimental category: | L |
PMID: | 18212065 |
Experiment: | Cell morphological analysis//SA-β-gal activity assay |
Description: | Notably,greater than 80% of Flag-TRF2△B△M-expressing fibroblasts showed a replicative senescence-like phenotype, exhibiting a flattened morphology, and stained positive for SA-β-gal activity. |
Target gene: | WRN |
Official symbol(s): | WRN |
R-AG-Target gene: | -- |
Subcategory: | Unclear |
Target gene experiment: | Western blot//SA-β-gal activity assay |
Target gene description: | Eight days after transduction, 70% of the wild-type WRN-complemented WS fibroblasts expressing Flag-TRF2△Bstained positive for SA-βgal, demonstrating that TRF2△B-induced cellular senescence was reconstituted by the expression of a functional WRN protein. In contrast, overexpression of Flag-TRF2△B failed to increase the percentage of SA-βgal-positive cells in the lines expressing WRN variants lacking helicase, exonuclase, or both activities. |
Regulatory pathway: | P53-P21 |
R-AG-Pathway: | Upregulation |
Official symbol(s): | TP53-CDKN1A |
Pathway experiment: | Western blot |
Pathway description: | Western blot analysis shows that p53 and its downstream target, p21, are upregulated in normal fibroblasts expressing Flag-TRF2△B△M. |
Annotation:
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