| Gene name: | MDC1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Knockdown//Western blot |
| Description: | We noted that upon more prolonged culturing [>2 wk], primary human fibroblasts expressing MDC1 shRNAs sh1 or sh2 started proliferating more slowly than the control cells and attained a senescence phenotype, even in the absence of TRF2-DN.in human cells the up-regulation of p53 and p21 in response to TRF2-DN was unaltered by MDC1 knockdown, and primary human fibroblasts underwent senescence within a week of TRF2 inhibition regardless of the level of MDC1. |
| Regulatory pathway: | ATM |
| R-AG-Pathway: | -- |
| Official symbol(s): | ATM |
| Pathway experiment: | Knockdown//RT-PCR//Western blot |
| Pathway description: | We found that this shRNA [sh3] has extensive sequence identity to the mRNA for the ATM kinase and induced a significant reduction in ATM protein levels. |
Annotation:
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