| Gene name: | IGF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Bone |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay |
| Description: | The senescent (SA-β-gal positive) MSCs showed a blight-blue staining in their central cytoplasm.It is noteworthy that the reduced proliferation, accumulative NCD and marker gene expressions, and the increased senescence were also observed in the void vector transfected MSCs after the selection, indicating that the unexpected cellular senescence may be, at least partly, due to a side effect of puromycin. |
| Target gene: | RAS |
| Official symbol(s): | RAS |
| R-AG-Target gene: | Activation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | In unselected cultures, no significant changes in activities of Ras(GTP-Ras/Total Ras) were observed in both void vector and IGF-1 transfected cells comparing to the parallel culture; but in the puromycin-selected cultures, the Ras activities in both void vector and IGF-I transfected MSCs were significantly increased with a higher level found in the void vector transfected cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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