| Gene name: | HSPA4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | TF1-L1FK clone A5 |
| Experiment: | MTT assay |
| Description: | We observed that AP20187 exerted a proliferative effect that was about ninefold higher in comparison with untreated and KNK437‐treated cells. In AP20187 plus KNK437‐treated cells, the proliferative response was markedly diminished in comparison with AP20187‐treated cells; however; the proliferative response in these cultures was significantly higher (about fourfold) in comparison with KNK437‐treated cells. |
| Target gene: | AKT |
| Official symbol(s): | AKT |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We analyzed lysates of A5 cells that were treated with AP20187 and KNK437 and immunoblotted using antibodies to Akt and Erk1/2. Akt was present in abundant levels in AP20187‐stimulated cells. When cells were coincubated with AP20187 and increasing concentrations of KNK437, a dose‐dependent decrease in Akt production was observed. This effect was less acute in comparison with cells treated with KNK437 alone. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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