| Gene name: | CDKN2A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Tumor tissue tissue |
| Cell name: | NP-9,NP-18,NP-29,NP-31 |
| Gene ID: | 1029 |
| Category: | protein coding |
| Phenotype: | Pancreatic cancer |
| Experimental category: | L |
| PMID: | 11687897 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | In three of the four cell lines assayed, cells acquired a more refractile cytoplasm and showed a flattened and enlarged morphology, characteristic of arrested or senescent cells.Results from these experiments clearly indicate that in Ad-p16–transduced NP-9 cells, DNA synthesis was stopped, whereas transduced NP-18 cells did not show variations in the percentage of cells in S phase. NP-29 and NP-31 cells did not show such a clear arrest in their cell cycle profile as NP-9 cells when transduced with Ad-p16. However, significant decreases in S phase percentage were observed in 5-BrdU incorporation experiments . Acidic β-gal staining indicated that NP-9, NP-29, and NP-31 cells were committed to a senescent phenotype, as assessed by the increase in their SA-β-gal activity. NP-18 cells did not show any increase in SA-β-gal activity, corroborating the different destination of these cells when p16 is overexpressed. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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