| Gene name: | DAXX |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | mOSE |
| Gene ID: | 1616 |
| Category: | protein coding |
| Phenotype: | Ovarian cancer |
| Experimental category: | HL |
| PMID: | 23542781 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//qRT-PCR |
| Description: | Morphological observations showed that cultured Daxxflox/flox mOSE cells were larger in size with flattened shapes after 15 days in culture, which is a feature of cellular senescence.This showed that 4% of cells hadundergone senescence after 4 days in culture, and >90% of cells were senescent after 15 days in culture. However, senescence (positive staining for SA-β-gal) was barely detectable in immortalized mOSE cells.p16, p21,and p27 mRNAs were highly expressed in Daxxflox/floxmOSE cells after culture for 1 day, whereas these were weakly expressed in immortalized mOSE cells.Senescence occurred rapidly for Daxxflox/floxmOSE cells at 24 h after infection. SA-β-Gal staining showed that about 35% of Daxxflox/floxmOSE cells infected with pAd-GFP-Cre were stained positively vs. about 10% of Daxxflox/floxmOSE cells that were infected with pAd-GFP as a control. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Immunofluorescence//qRT-PCR |
| Pathway description: | p21 and p53 accumulated in DAXX-deleted mOSE cells, which suggested that p21/p53 may play an essential role in DAXX deletion-induced senescence. Immunofluorescent staining showed that p27 expression in DAXX-deleted mOSE cells was significantly increased compared with that in control cells. Western blot and real-time PCR analyses showed that the expression of p27 (a cyclin-dependent kinase inhibitor) wa up-regulated in DAXX-deleted cells. However, NOXA and PUMA (p53 inducible genes) mRNA expression increased significantly in DAXX-deleted cells as shown by real-time PCR.DAXX-induced senescence appeared to be largely p21-dependent. |
Annotation:
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